| 基于邻苯二胺电聚合膜的抗体固定化方法研究 |
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| 引用本文： | 李继山,吴朝阳,王桦,沈国励,俞汝勤.基于邻苯二胺电聚合膜的抗体固定化方法研究[J].高等学校化学学报,2003,24(4):624-626. |
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| 作者姓名： | 李继山 吴朝阳 王桦 沈国励 俞汝勤 |
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| 作者单位： | 化学生物传感与计量学国家重点实验室, 湖南大学化学化工学院, 长沙 410082 |
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| 基金项目： | 国家自然科学基金 (批准号 :2 9975 0 0 6,2 0 0 75 0 0 6)，教育部博士点基金 (批准号 :2 0 0 10 5 32 0 0 8)资助 |
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| 摘 要： | Si等 1] 在压电石英晶体金电极表面先电聚合了一层聚苯胺膜 (PAn) ,再于 PAn膜上电聚合一层聚间苯二胺膜 (Pm PD) ,形成一双层膜 (Pm PD和 PAn) ,而后通过戊二醛共价键合固定化方法 ,实现对生物蛋白质分子的固定和对生物细胞的测定 .但在上述方法中 ,传感器难以再生且蛋白质分子的固定量较少 .参照文献 2 ],本文提出了一种在电聚合邻苯二胺薄膜上进行可逆的抗体固定化的新方法 .通过控制溶液的 p H值 ,在带正电的电聚合邻苯二胺膜表面先自组装一层聚阴离子聚苯磺酸根 (PSS)层 ,使传感器得到一个带负电的载体表面 ,再通过静电吸附 ,…
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| 关 键 词： | 电聚合邻苯二胺膜 PSS聚阴离子膜 自组装 压电免疫传感器 |
| 文章编号： | 0251-0790(2003)04-0624-03 |
| 收稿时间： | 2002-06-07 |
Studies on Antibody Immobilization Based on o-Phenylenediamine Electropolymerized Films for Immunosensors |
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| LI Ji Shan,WU Zhao Yang,WANG Hua,SHEN Guo Li ,YU Ru Qin.Studies on Antibody Immobilization Based on o-Phenylenediamine Electropolymerized Films for Immunosensors[J].Chemical Research In Chinese Universities,2003,24(4):624-626. |
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| Authors: | LI Ji Shan WU Zhao Yang WANG Hua SHEN Guo Li YU Ru Qin |
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| Institution: | State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemisty and Chemical Engineering, Hunan University, Changsha 410082, China |
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| Abstract: | A novel method based on electropolymerized films(Eps) is proposed for immobilizing antibodies(antigens) through a polyelectrolyte mediated layer. The o phenylenediamine Eps are deposited on the surfaces of quartz crystal microbalance(QCM) with an electropolymerized method using o phenylenediamine as the precursor. After self assembling a polystyrenesulfonate(PSS) layer on the Eps, the immobilization of goat anti IgG antibody, as an example, is investigated. The effects of experimental conditions about PSS self assembly, IgG antibody immobilization and immunoreaction et al. on the performances of immunosensor were discussed respectively. The QCM immunosensor can be used to quantitatively determine NHIgG in the range of 1.67-196.08 μg/mL. Moreover, the PSS and protein layer can easily be removed by shifting the pH, making the immunosensor regenerable. |
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| Keywords: | o Phenylenediamine electropolymerized films PSS polyanion film Self assembly Piezoelectric immunosensors |
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