Two-dimensional gel electrophoresis of selenized yeast and autoradiography of 75Se-containing proteins |
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Authors: | C C Chéry E Dumont R Cornelis L Moens |
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Institution: | Laboratory of Analytical Chemistry, Ghent University, Belgium. cyrille.chery@rug.ac.be |
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Abstract: | Two-dimensional high-resolution gel electrophoresis (2DE) has been applied to the fractionation of 75Se-containing proteins in yeast, grown in 75Se-containing medium, and autoradiography was used for detection of the 75Se-containing proteins. Gel filtration and ultrafiltration were used to check whether the selenium side-chains were stable in the presence of the chemicals used for lysis and 2DE. The mass distribution of the selenium-containing proteins was estimated by use of gel filtration and the results were compared with the distribution obtained by 2DE. A 2DE map of selenium-containing proteins in yeast is presented, and compared with a total protein map of yeast. |
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