Determination of domoic acid in Japanese mussels by enzyme immunoassay |
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Authors: | Kawatsu K Hamano Y Noguchi T |
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Institution: | Osaka Prefectural Institute of Public Health, Department of Food Microbiology, Nakamichi, Japan. |
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Abstract: | Ten samples of commercial blue mussels (Mytilus edulis) from Japan were analyzed for domoic acid by an indirect competitive enzyme immunoassay (idc-EIA) based on an anti-domoic acid monoclonal antibody. Domoic acid was found in all samples at low concentrations (0.11-1.81 ng/g mussel tissue). The presence of domoic acid was confirmed by liquid chromatography coupled with immunoaffinity chromatography using an anti-domoic acid monoclonal antibody as ligand. To our knowledge, this is the first reported detection of domoic acid, a causative agent of amnesic shellfish poisoning, in Japanese mussels. |
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