Different detection systems for the HPLC analysis of oxylipins, by-products of the enzymatic generation of 2E-hexenal |
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Authors: | Bettina Rehbock Dietmar Ganßer R G Berger |
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Institution: | Institut für Lebensmittelchemie, Universit?t Hannover, Wunstorfer Strasse 14, D-30453 Hannover, Germany e-mail: rg.berger@mbox.lci.uni-hannover.de, DE
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Abstract: | The incubation of 13-hydroperoxy-9Z,11E,15Z-octadecatrienoic acid (13-HPOT) with a hydroperoxide lyase containing extract of mung bean seedlings (Phaseolus radiatus L.) led not only to the formation of 2E-hexenal, but also to the generation of several non-volatile by-products (oxylipins). These oxylipins, generated by the catalysis of other 13-HPOT metabolizing enzymes, were analyzed by high performance liquid chromatography (HPLC) without time-comsuming derivatization procedures, which would be necessary for their volatilization and stabilization during gaschromatography (GC). Different detection systems such as an evaporative light-scattering detector (ELSD), diode-array detector (DAD), and particle beam-mass spectrometry (PB-MS) were applied. The utilization of an ELSD represented an improvement of sensitivity compared to a DAD, especially in the case of substances with low UV-activity. |
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