原位成形光学微透镜的聚光性能研究

为解决荧光谱微检测系统中光学微透镜制作的困难，提出一种原位成形微透镜的制作方法，研究该微透镜的聚光性能，利用传统几何光学原理，分别推导出激发微透镜与检测微透镜的光线追迹方程，用该方程计算了2种微透镜的照射点分布几率，画出相应的分布曲线图，讨论了不同形状参数的微透镜对聚光性能的影响。研究结果表明，通过在光学胶中适当掺杂及打磨基底玻璃，可减小接触角，加大微透镜的形状参数，既能坚固透镜与基底间的粘接强度，又能提高微透镜的聚光本领。制作了使用原位成形光学微透镜的荧光谱微检测器，对生物荧光试剂进行了测试。实验结果表明，使用原位成形光学微透镜进行聚光，可使生物荧光谱强度提高4倍以上，通过在光学胶中掺杂以及打磨玻璃的办法，可以提高透镜的聚光本领约1.2倍。

Light-gathering ability of optic micro-lens molded on needing position

In order to solve the difficulty for manufacture of micro-lens in micro-detector of fluorescent spectrum, a new manufacture technique of micro-lens, molded on needing position, was proposed. Light gathering ability of the optic micro-lens was researched. Using principles of traditional geometric optics, ray tracking equations of stimulating micro-lens and detecting micro-lens were derived. Distributed probabilities of illumination points for the two micro-lenses were calculated by using these equations. Some corresponding distributed curves were given. Influence of the micro-lenss shape on the light gathering ability was discussed. Results indicate that the contact angle between optic glue and glass base can be decreased and the shape parameter of the micro-lens can be increased by adulteration in the optic glue and milling the glass. It can both strengthen adhesiveness between the glue and the glass, and increase light gathering ability of the micro-lens. A micro-detector for fluorescent spectrum with micro-lens molded on needing position was manufactured and the biological fluorescent reagent was measured. Experiment results indicate that the intensity of the biologic fluorescent spectrum can be increased more than 4 times by using micro-lens molded on needing position, the light gathering ability of the micro-lens can be increased 1.2 times though adulteration in the glue and milling the glass.