| 制备反相高效液相色谱法用于从杉材中分离制备倍半萜类化合物 |
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| 引用本文： | 陈晓辉,张晖芬,毕开顺,金哲史,崛池道郎.制备反相高效液相色谱法用于从杉材中分离制备倍半萜类化合物[J].色谱,2005,23(1):85-87. |
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| 作者姓名： | 陈晓辉 张晖芬 毕开顺 金哲史 崛池道郎 |
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| 作者单位： | 1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;
2. Department of Bioresources Science, Faculty of Agriculture, Ehime University, Kochi 783-8502, Japan |
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| 摘 要： | 利用制备高效液相色谱法从日本产杉材(Cryptomeria japonica D.Don)中分离制备倍半萜类化合物。在YWC-Pack C18柱上,以异丙醇-甲醇-正己烷-水(体积比为50∶35∶10∶5)为流动相,流速为5 mL/min,制备了2种倍半萜类化合物,经紫外光谱、红外光谱及核磁、质谱分析,确认它们分别为(-)-cubebol和(+)-2,7(14),10-bisa-bolatrien-1-ol-4-one。所制备的两个化合物的纯度分别为98.7%和99.1%。
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| 关 键 词： | 倍半萜类化学成分 杉材 制备液相色谱 |
| 文章编号： | 1000-8713(2005)01-0085-03 |
| 收稿时间： | 2004-2-17 |
| 修稿时间： | 2004年2月22日 |
Isolation and Preparation of Sesquiterpenols from the Japanese Cedar, Cryptomeria japonica D. Don by Preparative Reversed-Phase High Performance Liquid Chromatography and Its Characterization |
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| CHEN Xiaohui,ZHANG Huifen,BI Kaishun,KIM Chul-Sa,HORIIKE Michio.Isolation and Preparation of Sesquiterpenols from the Japanese Cedar, Cryptomeria japonica D. Don by Preparative Reversed-Phase High Performance Liquid Chromatography and Its Characterization[J].Chinese Journal of Chromatography,2005,23(1):85-87. |
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| Authors: | CHEN Xiaohui ZHANG Huifen BI Kaishun KIM Chul-Sa HORIIKE Michio |
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| Institution: | 1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China;
2. Department of Bioresources Science, Faculty of Agriculture, Ehime University, Kochi 783-8502, Japan |
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| Abstract: | A method for the isolation of sesquiterpenols from the Japanese Cedar, Cryptomeria japonica D. Don by preparative reversed-phase high performance liquid chromatography (RP-HPLC) was established. The preparation of the sesquiterpenols was carried out on a preparative liquid chromatograph with a YWC-Pack C18 column, and the mobile phase was isopropanol-methanol-hexane-water (50:35:10:5, v/v) at a flow rate of 5.0 mL/min. The two sesquiterpenols, (-)-cubebol and (+)-2,7(14),10-bisabolatrien-1-ol-4-one, were separated by liquid chromatography and identified by spectroscopic analyses (ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS)). The purities of the two sesquiterpenols were 98.7% and 99.1%, respectively. |
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| Keywords: | preparative high performance liquid chromatography chemical constituents of sesquiterpenols Cryptomeria japonica D Don |
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