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Single-molecule spectroscopy of fluorescent proteins |
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| Authors: | Blum Christian Subramaniam Vinod | ||
| Institution: | (1) Biophysical Engineering Group, MESA+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands;(2) Biophysical Engineering Group, Institute for Biomedical Technology, Faculty of Science and Technology, MESA+ Institute of Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands | ||
| Abstract: | The discovery and use of fluorescent proteins has revolutionized cellular biology. Despite the widespread use of visible fluorescent
proteins as reporters and sensors in cellular environments the versatile photophysics of fluorescent proteins is still subject
to intense research. Understanding the details of the photophysics of these reporters is essential for accurate interpretation
of the biological and biochemical processes illuminated by fluorescent proteins. Some aspects of the complex photophysics
of fluorescent proteins can only be observed and understood at the single-molecule level, which removes averaging inherent
to ensemble studies. In this paper we review how single-molecule emission detection has helped understanding of the complex
photophysics of fluorescent proteins.
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| Keywords: | Bioanalytical methods Fluorescence Luminescence Laser spectroscopy Spectroscopy Instrumentation UV– visible | ||
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