Comparative studies of oxidation kinetics of benzidine and its derivatives in the presence of various hemoproteines |
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| Authors: | V P Kurchenko N V Gavrilenko S A Kotova and A T Pikulev |
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| Institution: | (1) V. I. Lenin Byelorussian State University, Minsk, USSR |
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| Abstract: | Kinetic parameters for the oxidation of benzidine, 3,3-dimethoxybenzidine, 3,3-dimethylbenzidine and 3,3,5,5-tetramethylbenzidine by horse-radish peroxidase and hemoglobin in the presence of hydrogen peroxide have been measured in citrate-acetate buffer at 30°C and pH=5.5. The mechanism of peroxidase oxidation of several benzidine derivatives by hemoproteines is discussed with respect to the number of substituents.
   30°C       -       5,5         , 3,3-, 3,3- 3,3,5,5--    .   .
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