Facile Fabrication of a Graphene‐based Electrochemical Biosensor for Glucose Detection |
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| Authors: | Yanqin Zhang Youjun Fan Shanshan Wang Yiliang Tan Xingcan Shen Zujin Shi |
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| Affiliation: | 1. Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, Guangxi 541004, China;2. Beijing National Laboratory for Molecular Sciences, State Key Lab of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
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| Abstract: | A simple and effective glucose biosensor based on immobilization of glucose oxidase (GOD) in graphene (GR)/Nafion film was constructed. The results indicated that the immobilized GOD can maintain its native structure and bioactivity, and the GR/Nafion film provides a favorable microenvironment for GOD immobilization and promotes the direct electron transfer between the electrode substrate and the redox center of GOD. The electrode reaction of the immobilized GOD shows a reversible and surface‐controlled process with the large electron transfer rate constant (ks) of 3.42±0.08 s?1. Based on the oxygen consumption during the oxidation process of glucose catalyzed by the immobilized GOD, the as‐prepared GOD/GR/Nafion/GCE electrode exhibits a linear range from 0.5 to 14 mmol·L?1 with a detection limit of 0.03 mmol·L?1. Moreover, it displays a good reproducibility and long‐term stability. |
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| Keywords: | graphene glucose oxidase direct electrochemistry biosensor electroanalysis |
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