Ligand Conformation Dictates Membrane and Endosomal Trafficking of Arginine‐Glycine‐Aspartate (RGD)‐Functionalized Mesoporous Silica Nanoparticles |
| |
Authors: | I‐Ju Fang Dr. Igor I. Slowing Dr. Kevin C.‐W. Wu Prof. Dr. Brian G. Trewyn |
| |
Affiliation: | 1. Department of Chemistry, Iowa State University, Ames, Iowa 50011 (USA), Fax: (+1)?515‐294‐4709;2. US Department of Energy, Ames Laboratory, Chemical and Biological Sciences Division, Ames, IA 50011 (USA);3. Current Address: Department of Chemical Engineering, National Taiwan University, Taipei (Taiwan) |
| |
Abstract: | Recent breakthrough research on mesoporous silica nanoparticle (MSN) materials has illustrated their significant potential in biological applications due to their excellent drug delivery and endocytotic behavior. We set out to determine if MSN, covalently functionalized with conformation specific bioactive molecules (either linear or cyclic RGD ligands), behave towards mammalian cells in a similar manner as the free ligands. We discovered that RGD immobilized on the MSN surface did not influence the integrity of the porous matrix and improved the endocytosis efficiency of the MSN materials. Through competition experiments with free RGD ligands, we also discovered a conformation specific receptor–integrin association. The interaction between RGD immobilized on the MSN surface and integrins plays an important role in endosome trafficking, specifically dictating the kinetics of endosomal escape. Thus, covalent functionalization of biomolecules on MSN assists in the design of a system for controlling the interface with cancer cells. |
| |
Keywords: | cell recognition endocytosis integrin mesoporous materials RGD |
|
|