Preparation of High‐Performance Water‐Soluble Quantum Dots for Biorecognition through Fluorescence Resonance Energy Transfer |
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| Authors: | Dr. Chin‐Ping Huang Cheng‐Fu Chao Mo‐Yuan Shen Prof. Dr. Teng‐Ming Chen Prof. Dr. Yaw‐Kuen Li |
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| Affiliation: | 1. Nanotechnology Research Center, Nano‐Instrumentation and Application Division, Industrial Technology Research Institute, 195, Sec. 4, Chung Hsing Rd., Chutung, Hsinchu, Taiwan 31040 (ROC);2. Department of Applied Chemistry, National Chiao Tung University, Science Building 2, 1001 Ta Hsueh Road, Hsinchu, Taiwan 300 (ROC), Fax: (+886)?3‐5723764 |
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| Abstract: | An improved method for the synthesis of high‐performance and water‐soluble quantum dots (QDs) involving the encapsulation of mercaptosuccinic acid coated QDs (MSA‐QDs) with poly(diallyldimethylammonium chloride) (PDDA) followed by their direct photoactivation with fluorescent radiation near 295 K to yield PDDA‐coated QDs (PDDA‐QDs) has been demonstrated. The quantum yield (QY) of the PDDA‐QDs was significantly improved from 0.6 (QY of MSA‐QDs) to 48 %. By using this synthetic strategy, highly photoluminescent PDDA‐QDs of varied size were readily prepared. The surface properties of PDDA‐QDs and MSA‐QDs were extensively characterized. The highly luminescent and positively charged PDDA‐QDs serve as a useful and convenient tool for protein adsorption. With a Δ5‐3‐ketosteroid isomerase adsorbed PDDA‐QD complex, the biorecognition of steroids was demonstrated through the application of fluorescent resonance energy transfer. |
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| Keywords: | FRET molecular recognition photoactivation quantum dots steroids |
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