Bacitracin‐Conjugated Superparamagnetic Iron Oxide Nanoparticles: Synthesis,Characterization and Antibacterial Activity |
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Authors: | Wenjing Zhang Xinhao Shi Dr Jing Huang Yixuan Zhang Prof Zirong Wu Prof Yuezhong Xian |
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Institution: | 1. Chemistry, East China Normal University, 3663 Zhongshan Road (N), Shanghai 200062 (P. R. China);2. School of Life Science, East China Normal University, 3663 Zhongshan Road (N), Shanghai 200062 (P. R. China) |
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Abstract: | Bacitracin‐conjugated superparamagnetic iron oxide (Fe3O4) nanoparticles were prepared by click chemistry and their antibacterial activity was investigated. After functionalization with hydrophilic and biocompatible poly(acrylic acid), water‐soluble Fe3O4 nanoparticles were obtained. Propargylated Fe3O4 nanoparticles were then synthesized by carbodiimide reaction of propargylamine with the carboxyl groups on the surface of the iron oxide nanoparticles. By further reaction with N3‐bacitracin in a CuI‐catalyzed azide–alkyne cycloaddition, the magnetic Fe3O4 nanoparticles were modified with the peptide bacitracin. The functionalized magnetic nanoparticles were characterized by powder X‐ray diffraction, X‐ray photoelectron spectroscopy, TEM, zeta‐potential analysis, FTIR spectroscopy and vibrating‐sample magnetometry. Cell cytotoxicity tests indicate that bacitracin‐conjugated Fe3O4 nanoparticles show very low cytotoxicity to human fibroblast cells, even at relatively high concentrations. In view of the antibacterial activity of bacitracin, the biofunctionalized Fe3O4 nanoparticles exhibit an antibacterial effect against both Gram‐positive and Gram‐negative organisms, which is even higher than that of bacitracin itself. The enhanced antibacterial activity of the magnetic nanocomposites allows the dosage and the side effects of the antibiotic to be reduced. Due to the antibacterial effect and magnetism, the bacitracin‐functionalized magnetic nanoparticles have potential application in magnetic‐targeting biomedical applications. |
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Keywords: | antibiotics click chemistry cytotoxicity magnetic properties nanoparticles |
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