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Nanowire Transistor‐Based Ultrasensitive Virus Detection with Reversible Surface Functionalization |
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| Authors: | Pei‐Ling Chiang Tzu‐Chi Chou Tzu‐Heng Wu Chang‐Chi Li Dr Chun‐Da Liao Dr Jeng‐Yu Lin Ming‐Hsueh Tsai Dr Chia‐Chang Tsai Chih‐Jung Sun Prof?Dr Ching‐Ho Wang Prof?Dr Jim‐Min Fang Prof?Dr Yit‐Tsong Chen |
| Institution: | 1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C), Fax: (+886)?2‐2363‐6359, Fax: (+886)?2‐2363‐7812;2. Institute of Atomic and Molecular Sciences, Academia Sinica, P. O. Box 23‐166, Taipei 106, Taiwan (R.O.C);3. Department of Veterinary Medicine, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C);4. The Genomics Research Center, Academia Sinica, P. O. Box 23‐166, Taipei 106, Taiwan (R.O.C) |
| Abstract: | We have applied a reusable silicon nanowire field‐effect transistor (SiNW‐FET) as a biosensor to conduct ultrasensitive detection of H5N2 avian influenza virus (AIV) in very dilute solution. The reversible surface functionalization of SiNW‐FET was made possible using a disulfide linker. In the surface functionalization, 3‐mercaptopropyltrimethoxysilane (MPTMS) was first modified on the SiNW‐FET (referred to as MPTMS/SiNW‐FET), with subsequent dithiothreitol washing to reduce any possible disulfide bonding between the thiol groups of MPTMS. Subsequently, receptor molecules could be immobilized on the MPTMS/SiNW‐FET by the formation of a disulfide bond. The success of the reversible surface functionalization was verified with fluorescence examination and electrical measurements. A surface topograph of the SiNW‐FET biosensor modified with a monoclonal antibody against H5N2 virus (referred to as mAbH5/SiNW‐FET) after detecting approximately 10?17 M H5N2 AIVs was scanned by atomic force microscopy to demonstrate that the SiNW‐FET is capable of detecting very few H5N2 AIV particles. |
| Keywords: | biosensors nanowires reversible surface functionalization viruses |