A Fluorescence‐Based Supramolecular Tandem Assay for Monitoring Lysine Methyltransferase Activity in Homogeneous Solution |
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Authors: | Dr. Mara Florea Dr. Srikanth Kudithipudi Dr. Ana Rei María José González‐Álvarez Prof. Dr. Albert Jeltsch Prof. Dr. Werner M. Nau |
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Affiliation: | 1. School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen (Germany), Fax: (+49)?421‐200‐3229;2. Institute of Biochemistry, Stuttgart University, 70569 Stuttgart (Germany);3. Present address: Dpto. Química Física, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid (Spain) |
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Abstract: | The demand for practical and convenient enzyme assays for histone lysine methyltransferases (HKMTs) emerges along with the rapid development of this young class of enzymes. A supramolecular reporter pair composed of p‐sulfonatocalix[4]arene (CX4) and the fluorescent dye lucigenin (LCG) has been used to monitor enzymatic trimethylation of lysine residues in peptide substrates. The assay affords a switch‐ON fluorescence response and operates in a continuous, real‐time, and label‐free fashion. The underlying working principle relies on the higher affinity of the macrocycle towards the trimethylated product of the enzymatic reaction as compared to the substrate, which allows the assay to be carried out in the product‐selective mode. The final product incorporates a trimethylammonium moiety, a known high‐affinity binding motif for CX4. Two substrates corresponding to the H3 N‐terminal tail, namely, S2 (RTKQTA RKSTG GKAP) and S6 (QTA RKSTG GS), were selected as model compounds for methylation with the Neurospora crassa Dim‐5 enzyme and investigated by the newly developed supramolecular tandem HKMTs assay. Only the longer substrate S2 underwent methylation in solution. The potential of the assay for inhibitor screening was demonstrated by means of inhibition studies with 1,10‐phenanthroline to afford an inhibition constant of (70±20) μM . |
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Keywords: | calixarenes enzyme assays fluorescent dyes indicator displacement methylation supramolecular chemistry transferases |
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