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Enzymatic Reaction in Droplets Manipulated with Liquid Dielectrophoresis |
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| Authors: | Dr. Momoko Kumemura Prof. Dominique Collard Dr. Satoko Yoshizawa Bernard Wee Prof. Shoji Takeuchi Prof. Hiroyuki Fujita |
| Affiliation: | 1. LIMMS/CNRS‐IIS, (UMI 2820), Institute of Industrial Science, The University of Tokyo, 4‐6‐1 Komaba, Meguro‐ku, Tokyo, 153‐8505 (Japan), Fax: (+81)3‐5452‐6250;2. LIMMS/CNRS‐IIS, (UMI 2820), The University of Tokyo, 4‐6‐1 Komaba, Meguro‐ku, Tokyo, 153‐8505 (Japan);3. Current address: CGM‐CNRS, 1 Avenue de la Terrasse Bat 24, 91190 Gif sur Yvette (France);4. Institute of Industrial Science, The University of Tokyo, 4‐6‐1 Komaba, Meguro‐ku, Tokyo, 153‐8505 (Japan) |
| Abstract: | Droplet generation and transportation for biological reactions are conducted with liquid dielectrophoresis (LDEP), forming two hundred picoliter droplets and aligning them in an open environment above the micro‐machined electrodes. The generation of the dielectrophoresis signals was critically examined to actuate droplets in biological solutions without excessive Joule heating. Enzymatic reactions between β‐galactosidase and fluorescein di‐β‐D ‐galactopyranoside were succeeded in manipulated droplets, which was confirmed by fluorescence imaging. These results allow us to propose the integration of LDEP actuation in high throughput biomolecular assays. |
| Keywords: | dielectrophoresis droplets enzyme catalysis fluorescent probes microreactors |