Voltammetric Detection of a Specific DNA Sequence of Avian Influenza Virus H5N1 Using HS‐ssDNA Probe Deposited onto Gold Electrode |
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| Authors: | Kamila Malecka Iwona Grabowska Jerzy Radecki Anna Stachyra Anna Góra‐Sochacka Agnieszka Sirko Hanna Radecka |
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| Affiliation: | 1. Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10‐747 Olsztyn, Poland;2. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02‐106 Warsaw, Poland |
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| Abstract: | The working principle of a genosensor is based on the mechanism of ion‐channel mimetic sensors. The analytical signals generated upon hybridization processes were recorded by a redox active marker [Fe(CN)6]3?/4? present in the sample solution using voltammetric techniques. The developed genosensor was suitable for determination of 20‐mer complementary oligonucleotide sequence, and also of the PCR products containing the complementary 20‐mer sequence in various positions, with detection limits in the 10 pM range. The noncomplementary 20‐mer oligonucleotide sequence as well as the PCR product without complementary region generated very weak response. The good discrimination of the position of the complementary part in the PCR products was observed. |
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| Keywords: | Electrochemical genosensor HS‐ssDNA probe PCR products Avian influenza virus |
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