A Simple and Sensitive Label‐free Immunoassay for Factor B Using Resonance Scattering Spectral Detection |
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| Authors: | Jishun Li Lili Xu Lisheng Wang Aihui Liang Zhiliang Jiang |
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| Affiliation: | 1. State Key Laboratory Cultivation Base for the Chemistry and Molecular Engineering of Medicinal Resources, Ministry of Science and Technology of China;2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, Guangxi Normal University, Guilin, Guangxi 541004, China |
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| Abstract: | In pH 7.2 Na2HPO4‐NaH2PO4 buffer solution and in the presence of PEG‐6000, goat‐anti‐human factor B (GABF) was combined with human factor B (BF) specifically, and aggregated to form immune complex particles that exhibited a resonance scattering (RS) peak at 400 nm. The laser scattering indicated that the average diameter of immune complex particles was 1320 nm. BF in the concentration range of 0.04 to 9.60 µg/mL was proportional to the resonance scattering intensity at 400 nm. Its regression equation was ΔI=33.61C+ 1.4, with a correlation coefficient of 0.9969, and a detection limit of 0.01 µg/mL BF. This label‐free resonance scattering spectral (RSS) method has been applied to the determination of BF in serum samples, and the results were in agreement with that of the immunoturbity. |
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| Keywords: | factor B immunocomplex particle resonance scattering spectral assay |
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