|
|||||
|
|
Enhancement of Enzymatic IDE Biosensor Response Using Gold Nanoparticles. Example of the Detection of Urea |
|
| Authors: | W Nouira A Maaref F Vocanson M Siadat J Saulnier F Lagarde N Jaffrezic‐Renault |
| Institution: | 1. Université de Lyon, Laboratoire des Sciences Analytiques, Université Claude Bernard, Lyon 1, 43 Boulevard du 11 novembre 1918, 69622 Villeurbanne, France;2. Laboratoire de Physique et Chimie des Interfaces, Faculté des Sciences de Monastir, Avenue de l'Environnement, 5019 Monastir, Tunisia;3. Université de Lyon, F‐42023 Saint‐Etienne, France;4. CNRS, UMR 5516, Laboratoire Hubert Curien, 42023 Saint‐Etienne, France;5. Université de Saint‐Etienne, Jean‐Monnet, F‐42023 Saint‐Etienne, France;6. LASC – ISEA, Université de Metz, 7 Rue Marconi, 57070 Metz, France |
| Abstract: | A new conductometric biosensor based on interdigitated electrodes (IDEs) has been developed for the detection of enzymatic substrates using gold nanoparticles (GNPs), synthesized bellowing the citrate process, with an average diameter of 23 nm and functionalized with urease using layer‐by‐layer technique. A detection limit of 100 µM of urea is obtained when cross‐linked urease is directly immobilized on top of the IDEs (interdigitated distance: 20 µm) whereas a detection limit of 2 µM is obtained when urease functionalized gold nanoparticles are deposited on the top of the IDEs. The use of gold nanoparticles allows the increase of the sensitivity of detection (from 10 µS/mM to 107 µS/mM) due to the decrease of the thickness of probed zone. |
| Keywords: | Conductometric biosensor Gold nanoparticles Layer‐by‐Layer Urease |