Voltammetric Sensing of Amino Acids in the Presence of Cu(II) in Acidic and Alkaline Solutions |
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Authors: | Rasa Pauliukaite Edilson M Pinto Aziz Amine Christopher M A Brett |
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Institution: | 1. Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, 3004–535 Coimbra, Portugal tel/fax: +351239835295;2. Faculty of Science and Techniques, University Hassan II Mohammedia, BP 146?Mohammedia 20650, Morocco |
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Abstract: | A number of amino acids have been determined at carbon film electrodes in the presence of copper. Strongly acidic, 0.1 M HCl, in the presence of 0.1 mM Cu(II), as well as alkaline, 0.1 M NaOH, solution permit successful measurement of individual amino acids, clearer separation between oxidation of Cu and Cu‐complexes occurring in alkaline solution. Electrochemical impedance showed that Cu(II) facilitates charge transfer, particularly in alkaline medium. Square wave voltammetry with preconcentration increased the response compared to linear sweep voltammetry. Protein hydrolysis rates were monitored through determination of amino acids produced by decomposition, injecting samples into alkaline electrolyte solution. |
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Keywords: | Amino acid determination Cu(II) complex Electrochemical impedance Protein hydrolysis |
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