| 体积排阻色谱和激光光散射对大豆蛋白热诱导聚集体的研究 |
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| 引用本文： | 李向红,华欲飞,王自东,裘爱泳,Steve Cui.体积排阻色谱和激光光散射对大豆蛋白热诱导聚集体的研究[J].分析化学,2007,35(2):251-254. |
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| 作者姓名： | 李向红 华欲飞 王自东 裘爱泳 Steve Cui |
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| 作者单位： | 江南大学食品学院,无锡,214036;北京科技大学材料科学与工程学院,北京,100083;Food Research Program, Agriculture and Agri-Food Canada, Guelph, Ont.,Canada N1G 5C9 |
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| 基金项目： | 国家自然科学基金
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江苏省自然科学基金 |
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| 摘 要： | 采用体积排阻色谱(SEC-HPLC)和激光光散射(LLS)研究了不同浓度和离子强度下大豆蛋白热诱导聚集体的分子量分布和粒径分布。在离子强度为0时,SEC-HPLC的结果表明,热处理后的蛋白溶液主要由3部分组成,即聚集体、中间体和未聚集部分。聚集体部分随着浓度增加而逐渐增加;LLS的结果表明:体系有不均一的粒径分布,且浓度增加时体系的平均粒径增加。上述样品在较高离子强度下加热时,SEC-HPLC和LLS的结果都证明溶液中的中间体部分逐渐消失。因此,控制浓度和离子强度可以制备不同性质的大豆蛋白聚集体。
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| 关 键 词： | 大豆蛋白 聚集体 体积排阻色谱 激光光散射 |
| 修稿时间： | 2006-05-312006-09-15 |
Studies of Heat-induced Soy Protein Aggregates by High Performance Size Exclusion Chromatography and Laser Light Scattering |
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| Li Xiang-Hong,Hua Yu-Fei,Wang Zi-Dong,Qiu Ai-Yong,Steve Cui.Studies of Heat-induced Soy Protein Aggregates by High Performance Size Exclusion Chromatography and Laser Light Scattering[J].Chinese Journal of Analytical Chemistry,2007,35(2):251-254. |
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| Authors: | Li Xiang-Hong Hua Yu-Fei Wang Zi-Dong Qiu Ai-Yong Steve Cui |
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| Institution: | 1 .School of Food Science and Engineering, Southern Yangtze University, Wuxi 214036;2. Material Science and Engineering School, University of Science and Technology Beijing, Beijing 100083 ;3.Food Research Program, Agriculture and Agri-Food Canada, Guelph, Ont. , Canada NI G 509 |
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| Abstract: | Heat-induced soy protein aggregates of different protein concentrations and ionic strength were analyzed by high performance size exclusion chromatography(SEC-HPLC) for molecular weight distribution and laser light scattering(LLS) for particle size distribution.At ionic strength of 0,SEC-HPLC results revealed that the heated solutions were composed of three major fractions,i.e.,aggregate,intermediate species and un-aggregated molecules,with aggregate fraction increased as the protein concentration increased.LLS analysis showed the inhomogeneous particle size distributions with the average hydrodynamic radius(Rh) increased.When soy protein solutions were heated at elevated ionic strength,the SEC-HPLC and LLS profiles changed substantially and the intermediate species disappeared gradually.The experiments suggested a way to prepare soy protein aggregates with controlled structures under certain protein concentration and ionic strength. |
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| Keywords: | Soy protein aggregate high performance size exclusion chromatography laser light scattering |
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