| 漆树漆酶的催化氧化作用 (ⅹⅢ)──Fe~(2+)离子对漆酶催化氧化反应的影响() |
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| 引用本文： | 涂楚桥,梁宏,王光辉.漆树漆酶的催化氧化作用 (ⅹⅢ)──Fe~(2+)离子对漆酶催化氧化反应的影响()[J].高等学校化学学报,1999(2). |
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| 作者姓名： | 涂楚桥 梁宏 王光辉 |
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| 作者单位： | 广西师范大学化学化工系，武汉大学化学学院 |
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| 摘 要： | 以５，６－二溴－２，３－二氰基氢醌为底物，在ｐＨ４．５０条件下，用分光光度法考察了ＦｅＳＯ４对漆树漆酶催化氧化反应的影响．结果表明，在本文实验条件下ＳＯ２－４离子对酶促反应的影响基本可以忽略，而Ｆｅ２＋离子对漆酶的催化氧化反应则有明显的竞争性抑制作用．研究证实，Ｆｅ２＋离子的抑制作用是通过它还原酶促反应的产物半醌自由基阴离子来实现的
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| 关 键 词： | 漆树漆酶，Fe~(2＋)离子，竞争性抑制作用，分光光度法 |
Catalyzed Oxidation by \%Rhus Vernicifera\% Laccase() \= Effects of Fe\+\{2+\} Ion on the
Catalytic Oxidation by Laccase |
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| TU Chu\|Qiao,LIANG Hong.Catalyzed Oxidation by \%Rhus Vernicifera\% Laccase() \= Effects of Fe\+\{2+\} Ion on the
Catalytic Oxidation by Laccase[J].Chemical Research In Chinese Universities,1999(2). |
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| Authors: | TU Chu\|Qiao LIANG Hong |
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| Abstract: | The effects of FeSO\-4 on the
oxidation of 5,6\|dibromo\|2,3\|dicyanohydroquinone(DDBQH\-2) catalyzed by \%Rhus
vernicifera\% laccase were studied spectrophotometryly under condition of pH 4\^50. The
results show that under the condition of experiments, SO\+\{2-\}\-4 ion has little effect on the
enzymatic reaction, but Fe\+\{2+\} ion has obvious inhibition, the nature of inhibition is
competitive type. The results demonstrate that the inhibition is realized through the reduction of
product of enzymatic reaction by Fe\+\{2+\} ion. |
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| Keywords: | Rhus vernicifera\% laccase Fe\+\{2+\} ion Competitive inhibition Spectrophotometry |
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