In vivo metabolism study of ginsenoside Re in rat using high-performance liquid chromatography coupled with tandem mass spectrometry |
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Authors: | Liu Yang Shunjun Xu Chunjin Liu Zhijun Su |
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Institution: | (1) Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou, 510120, China;(2) Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, 999078, China;(3) Zhejiang Chinese Medical University, Hangzhou, 310053, China; |
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Abstract: | Ginsenoside Re is one of the major the bioactive triterpene saponins in ginseng root, a well-known adaptogen in traditional
Chinese medicine. It is believed that the lead compound may be further developed into a promising new drug for preventing
hypertension and cardiovascular disease. To better understand the pharmacological activities of the component, an investigation
of its in vivo metabolism was necessary. In the present study, a high-performance liquid chromatography coupled with electrospray
ionization and quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-TOF-MS/MS) has been applied to discover and identify
the metabolites of ginsenoside Re in rat urine following intravenous and oral administration of the component, respectively.
The rat urine samples were collected and pretreated through C18 solid-phase extraction cartridges prior to analysis. Negative electrospray ionization mass spectrometry was used to discern
ginsenoside Re and its possible metabolites in urine samples. The metabolites were identified and tentatively characterized
by means of comparing molecular mass, retention time, and fragmentation pattern of the analytes with those of the parent compound,
ginsenoside Re. As a result, eleven and nine metabolites together with Re were detected and identified in rat urine collected
after intravenous and oral administration, respectively. A possible metabolic pathway of ginsenoside Re was also investigated
and proposed. Oxidation and deglycosylation were found to be the major metabolic processes of the constituent in rat.
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