| Modification of Nylon
Membrane Used for Affinity Adsorption |
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| 作者姓名： | Hong Yu GAN Zhen Hua SHANG Jun De WANG |
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| 作者单位： | Dalian Institute of Chemical Physics，Chinese Academy of Sciences，Dalian 116012 |
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| 摘 要： | Using microporous affinity membrane in separation has advantages of large specific area,low backpressure and fast separation ratel. This led to obtain high purity proteins invery short time and reduce the possibility of denaturation of biomelecules in largelysimplified separation process. Nylon membrane offers narrow pore size distributionand good mechanical rigidity. However, nylon membrane has low concentration ofprimary amino group and nonspecific adsorption of protein'. To solve these prob…
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Modification of Nylon Membrane Used for Affinity Adsorption |
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| Hong Yu GAN, Zhen Hua SHANG, Jun De WANG.Modification of Nylon
Membrane Used for Affinity Adsorption[J].Chinese Chemical Letters,2000,11(2):165-166. |
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| Authors: | Hong Yu GAN Zhen Hua SHANG Jun De WANG |
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| Abstract: | Nylon membrane was modified by binding with polyhydroxyl-containing materials to increase its hydrophilicity and reduce its nonspecific interaction with proteins. The effect of binding hydrophilic materials on amount of ligand bound-Cibacron Blue F3GA (CBF) was investigated. Experimental data showed that the amount of CBF bound can be increased significantly after binding of hydrophilic materials. |
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| Keywords: | Nylon membrane modification hydrophilic materials affinity adsorption |
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