(1) Institute for Cytobiology and Cytopathology, University of Marburg, Marburg, Germany;(2) Institute for Molecular Biology and Tumor Research, University of Marburg, Marburg, Germany
Abstract:
Background
Apyrases are divalent ion dependent tri- and dinucleotide phosphatases with different substrate specificity. The intracellular lysosomal apyrase LALP70 is also expressed as a splice variant (LALP70v) lacking a VSFASSQQ motif in the center of the molecule (aminoacids 287–294). However, the functional significance of this motif is unknown. In this report we used a thin layer chromatography approach to study separately the UTPase and UDPase activity of the two LALP-enzymes.
