A fluorimetric and circular dichroism study of hemoglobin--effect of pH and anionic amphiphiles |
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Authors: | De Swati Girigoswami Agnishwar |
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Institution: | Department of Chemistry, University of Kalyani, Kalyani 741235, India. swati_de1@rediffmail.com |
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Abstract: | In this work, bovine hemoglobin (Hb) has been studied mainly by the fluorescence method. pH has been found to exert a profound effect on Hb structure. This has been confirmed by fluorescence and circular dichroism (CD) studies. The pH-induced change in quaternary structure of Hb indirectly affects its secondary structure. This in turn affects ligand binding to Hb at various pH. The binding of two amphiphiles, a bile salt and a surfactant, have been investigated. The pH-induced structural modification of Hb has been confirmed by studies with the well-known denaturant urea and the polarity probe ANS, which has been used as an extrinsic fluorophore. |
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