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Uricase based methods for determination of uric acid in serum |
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| Authors: | Yunsheng Zhao Xiaoyan Yang Wei Lu Hong Liao Fei Liao |
| Institution: | 1. Unit of Biochemical Pharmacology and Protein Biotechnology, Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing, 400016, People’s Republic of China 2. Department of Clinic Laboratory, The First Hospital of Qinhuangdao, Qinhuangdao, 066000, Hebei, People’s Republic of China 3. Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing, 400016, People’s Republic of China |
| Abstract: | The most common methods for determination of uric acid in serum are based on the use of the enzyme uricase. Uric acid is enzymatically oxidized by oxygen to produce hydrogen peroxide, allantoin, and carbon dioxide. The four most often applied uricase methods are critically compared in this review. |
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