Simultaneous determination of flavonoids and phenolic acids in Chinese herbal tea by beta-cyclodextrin based capillary zone electrophoresis |
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Authors: | Langzhu Chi Zicheng Li Shuqing Dong Pingang He Qingjiang Wang Yuzhi Fang |
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Institution: | 1. Department of Chemistry, East China Normal University, Shanghai, 200062, China
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Abstract: | Kaempferol, apigenin, rutin, quercetin, luteolin and ferulic acid are separated and detected in Chinese herbal tea using capillary zone electrophoresis coupled to amperometric detection. The phosphate running buffer also contains ß-cyclodextrin (ß-CD), which assists in separation and gives excellent separations within 20 min and detection limits as low as 10 ng mL?1 (S/N = 3). The effects of working electrode potential, pH and concentration of running buffer, concentration of ß-CD, separation voltage and injection time were investigated. The method was applied to analyze tea samples with recoveries in the range of 90.0 to 107.0%. The method offers high separation efficiency, short analysis time, small sample consumption, and good repeatability. |
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