| 离子色谱法测定饲料中氯化胆碱和三甲胺的含量 |
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| 引用本文： | 丁永胜,牟世芬. 离子色谱法测定饲料中氯化胆碱和三甲胺的含量[J]. 色谱, 2004, 22(2): 174-176 |
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| 作者姓名： | 丁永胜 牟世芬 |
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| 作者单位： | 1. 中国科学院生态环境研究中心,北京,100085;北京大学药学院化学生物系,北京,100083
2. 中国科学院生态环境研究中心,北京,100085 |
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| 摘 要： | 建立了离子色谱法测定饲料中氯化胆碱含量及鉴别饲料中氯化胆碱及掺假物三甲胺的方法。选用IonPac CS12阳离子交换色谱柱(250 mm×4 mm i.d.)和8.5 mmol/L H2SO4淋洗液,抑制型电导检测,在16 min内分离测定了包括胆碱和三甲胺在内的8种阳离子。胆碱和三甲胺的最小检出限分别为0.1 mg/L和0.05 mg/L。方法回收率为99.25%~102.5%。该方法具有灵敏度高、选择性强、操作简单等优点。
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| 关 键 词： | 离子色谱 氯化胆碱 三甲胺 饲料 |
| 文章编号： | 1000-8713(2004)02-0174-03 |
| 修稿时间： | 2003-06-11 |
Determination of Choline Chloride and Trimethylamine in Feedstuff by Ion Chromatography |
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| DING Yongsheng. Determination of Choline Chloride and Trimethylamine in Feedstuff by Ion Chromatography[J]. Chinese journal of chromatography, 2004, 22(2): 174-176 |
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| Authors: | DING Yongsheng |
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| Affiliation: | Research Center for Eco-Environmental Sciences, The Chinese Academy of Sciences, Beijng 100085, China. |
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| Abstract: | A method was developed for the determination of choline chloride (CC) and trimethylamine (TMA) in feedstuff by ion chromatography. The separation of eight ions including Li+, Na+, NH4+, K+, TMA, choline, Mg2+ and Ca2+ was achieved by using an IonPac CS12 column (250 mm x 4 mm i.d.) and 8.5 mmol/L H2SO4 as eluent. Cations were detected by suppressed conductivity detection. The limits of detection of CC and TMA were 0.1 mg/L and 0.05 mg/L, respectively. The method recoveries were between 99.25% and 102.5%. The method was sensitive, selective, and simple. The results of sample analysis showed that the method was very useful for the authenticating of choline chloride in feedstuffs. |
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| Keywords: | ion chromatography choline chloride trimethylamine feedstuffs |
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