NMR conformational analysis of biosynthetic precursor-type lipid A: monomolecular state and supramolecular assembly |
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Authors: | Oikawa Masato Shintaku Tetsuya Fukuda Naohiro Sekljic Harald Fukase Yoshiyuki Yoshizaki Hiroaki Fukase Koichi Kusumoto Shoichi |
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Affiliation: | Department of Chemistry, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan. |
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Abstract: | The detailed conformational analysis of a single molecule of the tetraacyl biosynthetic precursor-type lipid A and its characteristic supramolecular assembly in aqueous SDS-micelles are described. Regular molecular arrangements were observed by detailed analysis of the NMR spectra of synthetically pure specimens, including regiospecifically 13C-labeled ones. NMR analysis of a biologically inactive precursor-type analogue with four shorter acyl chains demonstrated its conformational flexibility, indicating the importance of hydrophobic interactions for maintaining the conformation of such molecules. |
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