| 生物除磷污泥胞外多聚物含磷形态的核磁共振分析 |
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| 引用本文： | 张志超,黄霞,杨海军,肖康,罗小,沙恒,陈奕名. 生物除磷污泥胞外多聚物含磷形态的核磁共振分析[J]. 光谱学与光谱分析, 2009, 29(2). DOI: 10.3964/j.issn.1000-0593(2009)02-0536-04 |
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| 作者姓名： | 张志超 黄霞 杨海军 肖康 罗小 沙恒 陈奕名 |
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| 作者单位： | 清华大学环境科学与工程系,环境模拟与污染控制国家重点联合实验室,北京,100084;清华大学化学系,北京,100084 |
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| 基金项目： | 国家杰出青年科学基金 |
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| 摘 要： | 利用31P-NMR研究了生物除磷污泥胞外多聚物(EPS)的含磷形态,并对检测方法中的影响因素进行了分析.研究发现,测定的三种污泥EPS中磷的形态有五种,磷酸盐、磷单脂、DNA磷、聚磷末端、焦磷酸盐和聚磷.具有除磷能力的A/A/O和A/A/O-MBR污泥其EPS中主要含的是聚磷,证明了EPS中含磷不仅是依靠吸附磷酸盐或生物聚磷过程磷酸盐的滞留,而且EPS本身可能也参与了生物聚磷过程.同时实验证明,EPS提取过程会对EPS含磷形态产生影响,但提取时间在1h内时影响较小;EPS在储存前需要进行中和,从而避免储存过程不同磷形态之间的转化.EPS含磷形态的31P-NMR测定过程中,EPS样品的pH 值对峰的分离和各峰化学位移的稳定性有较大影响,需要控制样品pH＞13.0以确保测定过程的准确性.
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| 关 键 词： | 胞外多聚物 核磁共振磷谱 生物除磷 磷形态 |
Study on P Forms in Extracellular Polymeric Substances in Enhanced Biological Phosphorus Removal Sludge by ~(31)P-NMR Spectroscopy |
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| ZHANG Zhie-hao,HUANG Xia,YANG Hai-jun,XIAO Kang,LUO Xiao,SHA Heng,CHEN Yi-ming. Study on P Forms in Extracellular Polymeric Substances in Enhanced Biological Phosphorus Removal Sludge by ~(31)P-NMR Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2009, 29(2). DOI: 10.3964/j.issn.1000-0593(2009)02-0536-04 |
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| Authors: | ZHANG Zhie-hao HUANG Xia YANG Hai-jun XIAO Kang LUO Xiao SHA Heng CHEN Yi-ming |
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| Affiliation: | ZHANG Zhi-chao 1,HUANG Xia 1,YANG Hai-jun 2,XIAO Kang 1,LUO Xiao 2,SHA Heng 1,CHEN Yi-ming 1 1. ESPC State Key Joint Laboratory,Department of Environmental Science , Engineering,Tsinghua University,Beijing 100084,China 2.Department of Chemistry,China |
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| Abstract: | The present paper investigated phosphorus forms in the extracellular polymeric substances(EPS) using 31 P-NMR spectroscopy,and analyzed the influential parameters for this method.The results showed that in the three investigated EPS samples five P forms were identified,including orthophosphate,orthophosphate monoesters,DNA,pyrophosphate and polyphosphate.And in the EPS extracted from A/A/O sludge and A/A/O-MBR sludge polyphosphate was the main phosphorus form.This indicated that P is contained in the EPS no... |
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| Keywords: | 31 P-NMR spectroscopy Extracellular polymeric substances Biological phosphorus removal Phosphorus form |
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