| 高效液相色谱法测定红茶中的茶黄素 |
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| 引用本文： | 王坤波,刘仲华,黄建安,龚雨顺.高效液相色谱法测定红茶中的茶黄素[J].色谱,2004,22(2):151-153. |
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| 作者姓名： | 王坤波 刘仲华 黄建安 龚雨顺 |
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| 作者单位： | 湖南农业大学,茶学教育部重点实验室,湖南省天然产物工程技术研究中心,湖南,长沙,410128 |
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| 基金项目： | 国家计委"十五"高新技术产业化重大专项资助项目(GJGX 02105 03). |
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| 摘 要： | 建立了一种用于分析红茶中茶黄素的高效液相色谱法。该方法采用反相C18柱;流动相A为2%醋酸,流动相B为乙腈-乙酸乙酯(体积比为21∶3),梯度洗脱,流速0.8 mL/min;紫外检测波长280 nm;柱温40 ℃;外标法定量。结果表明,所采用的标准曲线有良好的线性关系(r=0.9990~0.9992),加标回收率为96.88%~103.57%,方法的精密度良好(平均RSD<1.5%)。该方法简便、快速、准确,可用于实际样品的测定。
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| 关 键 词： | 茶黄素 高效液相色谱法 红茶 |
| 文章编号： | 1000-8713(2004)02-0151-03 |
| 修稿时间： | 2003年5月12日 |
Determination of Theaflavins in Black Tea by High Performance Liquid Chromatography |
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| Kunbo Wang,Zhonghua Liu,Jian''an Huang,Yushun Gong.Determination of Theaflavins in Black Tea by High Performance Liquid Chromatography[J].Chinese Journal of Chromatography,2004,22(2):151-153. |
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| Authors: | Kunbo Wang Zhonghua Liu Jian'an Huang Yushun Gong |
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| Institution: | Hunan Engineering & Technology Center for Natural Products, Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha 410128, China. wangkunbo@21cn.com |
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| Abstract: | A high performance liquid chromatographic method for the determination of theaflavins has been established. Separation was carried out on an ODS column (Shim-pack VP-ODS column, 4.6 mm i.d. x 150 mm) at 40 degrees C with a linear gradient elution of acetonitrile-ethyl acetate (21:3, v/v) and aqueous acetic acid (2%), and UV detection at 280 nm. The experimental results showed a good linear relationship between the peak area and the concentration of theaflavins (r = 0.9990-0.9992). The average recoveries (n = 5) for theaflavin products were 96.88%-103.57%. The average relative standard deviation was less than 1.5%. The results show that the method is simple, rapid and accurate for the determination of theaflavins in black tea and theaflavin extract. |
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| Keywords: | high performance liquid chromatography theaflavins black tea |
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