Beijing synchrotron radiation total-reflection X-ray fluorescence analysis facility and its applications on trace element study of cells |
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| Institution: | 1. Institute of Electrophysics, UB RAS, 106 Amundsen St., 620016 Ekaterinburg, Russia;2. Ural Federal University, 19 Mira St., 620002 Ekaterinburg, Russia;3. Institute of High Temperature Electrochemistry, UB RAS, 20 Academicheskaya St., 620137 Ekaterinburg, Russia;4. Institute of Metallurgy, UB RAS, 101 Amundsen St., 620016 Ekaterinburg, Russia |
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| Abstract: | The feasibility of total-reflection X-ray fluorescence (TXRF) analysis excited by synchrotron radiation applied to trace element analysis of biological cells is investigated. The Beijing synchrotron radiation TXRF facility and the related experimental method are also described. The elemental minimum detection limits of some standard reference materials are determined. The elemental compositions of a cluster of small intestine cells of a small white mouse are given, and hence the average trace element contents of the single small intestine cell are also obtained. With this technique, the changes of some trace elements in the cells of lung and cervix cancer before and after apoptosis are also preliminarily studied. |
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