On-chip pH measurement using functionalized gel-microbeads positioned by optical tweezers |
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Authors: | Maruyama Hisataka Arai Fumihito Fukuda Toshio |
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Affiliation: | Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan. maruyama@robo.mein.nagoya-u.ac.jp |
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Abstract: | This paper demonstrates local pH measurement in a microchip using a pH-sensing gel-microbead. To achieve this, the gel-microbead made of a hydrophilic photo-crosslinkable resin was functionalized with the pH indicator bromothymol blue (BTB). The primary constituent of this photo-crosslinkable resin is poly(ethylene glycol). Gel-microbeads impregnated with BTB were obtained by stirring the mixture solution, which was composed of the resin, BTB, and an electrolyte solution. The gel-microbead is polymerized by UV illumination. The polymerized gel-microbead can be manipulated by optical tweezers and made to adhere to a glass surface. The local pH was measured from the color of the gel-microbead impregnated with BTB by calibrated color information in the YCrCb color space. We succeeded in measuring the local pH value using the pH-sensing gel-microbead by manipulating and positioning it at the desired point in the microchip. |
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