| H9N2亚型禽流感病毒细胞适应株细胞致病性和HA、NA基因序列分析 |
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| 引用本文： | 牛学锋,罗琴芳,郭霄峰. H9N2亚型禽流感病毒细胞适应株细胞致病性和HA、NA基因序列分析[J]. 武汉大学学报(理学版), 2005, 0(Z2) |
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| 作者姓名： | 牛学锋 罗琴芳 郭霄峰 |
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| 作者单位： | 华南农业大学兽医学院 广东广州510642 |
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| 基金项目： | 国家自然科学基金资助项目(30270987,30440010)，广东省自然科学基金资助项目(020996) |
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| 摘 要： | 对分离自中国南方的H9N2亚型禽流感病毒A/Chicken/Guangxi/KMⅢ/99(H9N2)进行了MDCK细胞的连续传代和NA基因序列的初步分析.研究发现,病毒接种细胞36 h左右出现细胞病变(CPE),细胞肿胀变圆,聚集,脱落.流感病毒经MDCK细胞连续传代19代后,HA效价与亲代病毒相当.将传代获得的第19代病毒进行基因克隆,与亲代病毒进行序列比较,发现经传代后其HA1羧基端的分子特征是:R-S-S-R,仍属于非低致病力毒株.
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| 关 键 词： | 禽流感病毒 MDCK细胞 HA基因 NA基因 |
Pathogenicity and HA,NA Genes Sequences Analysis of Avian Influenza Virus H9N2 Subtype Adapted in Mammalian Cell Line |
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| NIU Xue-feng,LUO Qing-fang,GUO Xiao-feng. Pathogenicity and HA,NA Genes Sequences Analysis of Avian Influenza Virus H9N2 Subtype Adapted in Mammalian Cell Line[J]. JOurnal of Wuhan University:Natural Science Edition, 2005, 0(Z2) |
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| Authors: | NIU Xue-feng LUO Qing-fang GUO Xiao-feng |
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| Abstract: | The avian influenza virus was adapted to MDCK cell line by continuous passage,and the HA,NA gene sequences were analyzed.The study demonstrated that typical cytopathic effect(CPE) were seen,the HA titer was 9 log2,equal to the parental virus.The HA and NA gene showed 98%-99% nucleotide sequence homology between the variant and the parental isolate.Genetic analysis showed that the virus had the same amino acid near the HA connecting peptide: R-S-S-R. |
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| Keywords: | avian influenza virus MDCK cell line HA gene NA gene |
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