514— Oxidase-cofactor electrodes aimed at the quantitative measure of substrate concentrations

Two approaches are described briefly for utilizing oxidase enzymes in electrochemical sensors. In one approach an oxidase enzyme flavin cofactor was covalently coupled to the electrode surface with the anticipated readout being a current proportional to the concentration of enzyme substrate. In the second approach enzyme glocose oxidase was immobilized on platinum such that the potential of the platinum varied with the concentration of glucose in a solution buffered at pH 7.4. The status of the two projects is described.