Development and trends of biosurfactant analysis and purification using rhamnolipids as an example |
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Authors: | M Heyd A Kohnert T-H Tan M Nusser F Kirschhöfer G Brenner-Weiss M Franzreb S Berensmeier |
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Institution: | (1) Water Technology & Geotechnology Division, Institute for Technical Chemistry, Forschungszentrum Karlsruhe GmbH, 76021 Karlsruhe, Germany |
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Abstract: | During the last few decades, increasing interest in biological surfactants led to an intensification of research for the cost-efficient
production of biosurfactants compared with traditional petrochemical surface-active components. The quest for alternative
production strains also is associated with new demands on biosurfactant analysis. The present paper gives an overview of existing
analytical methods, based on the example of rhamnolipids. The methods reviewed range from simple colorimetric testing to sophisticated
chromatographic separation coupled with detection systems like mass spectrometry, by means of which detailed structural information
is obtained. High-performance liquid chromatography (HPLC) coupled with mass spectrometry currently presents the most precise
method for rhamnolipid identification and quantification. Suitable approaches to accelerate rhamnolipid quantification for
better control of biosurfactant production are HPLC analysis directly from culture broth by adding an internal standard or
Fourier transform infrared attenuated total reflectance spectroscopy measurements of culture broth as a possible quasi-online
quantification method in the future. The search for alternative rhamnolipid-producing strains makes a structure analysis and
constant adaptation of the existing quantification methods necessary. Therefore, simple colorimetric tests based on whole
rhamnolipid content can be useful for strain and medium screening. Furthermore, rhamnolipid purification from a fermentation
broth will be considered depending on the following application. |
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Keywords: | Rhamnolipids Glycolipids Biosurfactants Pseudomonas aeruginosa Analysis Purification |
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