| 从微生物中提取超氧化物歧化酶的研究 |
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| 引用本文： | 胡佩. 从微生物中提取超氧化物歧化酶的研究[J]. 四川师范大学学报(自然科学版), 1995, 18(5): 6-9 |
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| 作者姓名： | 胡佩 |
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| 作者单位： | 四川师范大学化学系 |
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| 摘 要： | 超氧化物歧化酶，简称ＳＯＤ（Ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ），是一种应用价值大、广泛存在于生物体细胞内的金属酶．本实验以酿酒酵母和嗜热脂肪芽孢杆菌为出发菌株，进行抗Ｈ_２Ｏ_２性能的筛选，选出耐受性好的菌株经２Ｌ发酵罐发酵后，提取到了热稳定性好的Ｃｕ，Ｚｎ－ＳＯＤ和Ｆｅ－ＳＯＤ，酶活力为３２９２和３５４７ｕ／ｍＬ，其比活力为３４５７和３０８４ｕ／ｍｇ．
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| 关 键 词： | 酿酒酵母 SOD 微生物 |
THE STUDY ON EXTRACTING SUPEROXIDE DISMUTASE FROM MICROORGANISMS |
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| Hu Pei. THE STUDY ON EXTRACTING SUPEROXIDE DISMUTASE FROM MICROORGANISMS[J]. Journal of Sichuan Normal University(Natural Science), 1995, 18(5): 6-9 |
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| Authors: | Hu Pei |
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| Abstract: | Superoxide dismutase(abbreviation SOD)are highly-valued metal enzymes which exist in most cell of organisms.The Saccharomyces Cerevisiae and Bacillus Stearothermophilus are used as primitive strains to screen.good strains with high H_2O_2 concentration resistance,which are cultivated in 2L fermentor,extracting Cu,Zn-SOD and Fe-SOD with fine heat stability.The enzyme activity of Cu,Zn-SOD and Fe-SOD are 3292 and 3547u/mL,the specific activity of them are 3457 and 3084 u/mg protein respectively. |
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| Keywords: | Saccharomyces Cerevisiae Bacillus Stearothermophilus Superoxide dismutase Extracting |
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