Surface modification of gold nanorods using layer-by-layer technique for cellular uptake |
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Authors: | Hironobu Takahashi Takuro Niidome Takahito Kawano Sunao Yamada Yasuro Niidome |
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Institution: | (1) Department of Materials Physics and Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan;(2) Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan;(3) Center for Future Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan |
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Abstract: | Gold nanorods (NRs), rod-shaped gold nanoparticles, were modified with bovine serum albumin (BSA) and polyethylenimine (PEI)
using layer-by-layer technique. From absorption spectroscopy and zeta potential measurements, it was obvious that NRs were
wrapped with these polymers without aggregation of NRs. Following BSA modification, the surface-modified NRs (BSA-NRs) were
well-dispersed without aggregation in biochemical conditions, verified from absorption spectroscopy. Further modification
with PEI provided positively charged NRs (PEI-NRs). A transmission electron microscopy image of PEI-NRs revealed that the
surface modification did not affect changing the shape of the initial NRs. In addition, the PEI-NRs retained the colloidal
stability of BSA-NRs in biochemical conditions. We have evaluated that transfection activity of PEI-NRs with HeLa cells. From
results of gene expression experiments, it was obvious that the stabilization of NRs by BSA and further modification with
PEI realized transfection using NRs into cultured cells. Moreover, the cellular uptake of NRs enabled cellular imaging using
light scattering from the NRs. |
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Keywords: | Gold nanorods Gold nanoparticles Gene delivery Layer-by-layer deposition Light scattering Colloids |
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