| 同步荧光分析法同时测定叶绿素a和叶绿素b |
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| 引用本文： | 黄贤智,许金钩,蔡挺.同步荧光分析法同时测定叶绿素a和叶绿素b[J].高等学校化学学报,1987,8(5):418. |
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| 作者姓名： | 黄贤智 许金钩 蔡挺 |
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| 作者单位： | 厦门大学化学系 |
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| 摘 要： | 众所周知,叶绿素a和叶绿素b(分别用Chla和Chlb表示)在绿色植物的光合作用中起着极重要的作用。它们的测定通常用于不同波长处测量吸光度或在不同激发-发射波长下测量荧光强度,而后解联立方程的光度法。
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| 收稿时间： | 1985-10-29 |
Simultaneous Determination of Chlorophyll a and Chlorophyll b by Synchronous Fluorimetry |
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| Huang Xianzhi,Xu Jingou,Cai Ting.Simultaneous Determination of Chlorophyll a and Chlorophyll b by Synchronous Fluorimetry[J].Chemical Research In Chinese Universities,1987,8(5):418. |
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| Authors: | Huang Xianzhi Xu Jingou Cai Ting |
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| Institution: | Department of Chemistry, Xiamen University, Xiamen |
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| Abstract: | This paper reports a method for simultaneous determination of chlorophyll a and chlorophyll b by synchronous fluorimetry. The scanning wavelength intervals (△λ) of 258nm and 193nm were used and the wavelengths of maximum emission in synchroneous spectra were at 670nm and 654nm for chlorophyll a and chlorophyll b, respectively. This method is simple and rapid, and the ranges of determination were- 0.5-200 ppb. and 0.5-150 ppb, for chlorophyll a and chlorophyll b, respectively. Accuracy of deicrmination was about 5%. |
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