Investigations on the interferon-induced 2'-5' oligoadenylate system using analytical capillary isotachophoresis |
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Authors: | G Bruchelt M Buedenbender J Treumer D Niethammer K Schmidt |
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Affiliation: | Children's Hospital, Department of Hematology, University of Tuebingen, F.R.G. |
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Abstract: | The activity of the interferon inducible enzyme 2'-5' oligoadenylate synthetase (2-5A synthetase, E.C. 2.7.7) which converts ATP into a series of 2'-5' oligoadenylates was measured using analytical capillary isotachophoresis. The turnover rate of ATP during the reaction was monitored by determination of its concentration at the beginning and the end of the 2-5A synthetase reaction. The enzyme was analysed in extracts of peripheral blood mononuclear cells either pretreated or not with interferon. |
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