傅里叶变换红外光谱法研究HL-60细胞的分化过程

利用傅里叶变换红外光谱法(FTIR)研究了人白血病细胞HL-60在全反式维甲酸(ATRA)作用下向粒细胞分化的过程。结果表明，分化后的HL-60细胞FTIR图谱发生了显著变化，体现在与蛋白质、脂类、核酸和多糖等生物大分子相关的特征性谱带上。细胞内脂类物质烃链增长，含量增加。指纹图谱区域(900～1 300 cm-1)的谱带随着分化进程呈现规律性的变化。其中，核酸含量相对增加，并在1 052和1 153 cm-1附近出现了新的谱峰，二阶导数谱进一步发现在1 022 cm-1处出现新峰，这说明蛋白糖基化，磷酸化以及核酸氢键加强作用在HL-60细胞分化的过程中起着重要作用。通过计算一些图谱参数，并与硝基四唑氮蓝(NBT)还原实验相比较，证明红外图谱的变化与分化程度成正相关。

Monitoring All-Trans-Retinoic Acid-Induced Differentiation of HL-60 Cells by Fourier-Transform Infrared Spectroscopy

This study reports a new in vitro analytical method, based on Fourier-transform infrared (FTIR) spectroscopy, to monitor the myeloid differentiation process in human myeloblast leukemia HL-60 cells induced by all-trans-retinoic acid (ATRA). The (alteration) of characteristic bands was identified in the differentiated cells, arising from proteins, lipids, carbohydrates and nucleic (acids). Besides the changes in lipid content and plasma membrane fluidity, the most striking changes were observed in the region of (nucleic) (acids) and carbohydrates. The authors speculate that the glycosylation and phosphorylation of proteins and the hydrogen-bonding of (nucleic) acids were involved in differentiation. The spectral parameters were correlated with the differentiation index, as determined by NBT (reduction) assay. These results suggest that FTIR spectroscopy can be used to monitor the differentiation process of HL-60 cells.