|
|||||
|
|
Laser energy dependence on femtosecond laser-induced nucleation of protein |
|
| Authors: | Hiroshi Y. Yoshikawa Ryota Murai Syou Maki Tomoya Kitatani Shigeru Sugiyama Gen Sazaki Hiroaki Adachi Tsuyoshi Inoue Hiroyoshi Matsumura Kazufumi Takano Satoshi Murakami Takatomo Sasaki Yusuke Mori |
| Affiliation: | 1. Division of Electrical, Electronic and Information Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan 2. SOSHO project (Crystal Design Project), Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan 3. CREST, JST, 4-7-8 Honcho, Kawaguchi, Saitama, 332-0032, Japan 4. SOSHO Inc., 1-6-18, Honmachi, Chuo-ku, Osaka, 541-0053, Japan 5. Department of Materials Chemistry, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan 6. Department of Material and Life Science, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan 7. Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan |
| Abstract: | We have investigated femtosecond laser-induced nucleation of hen egg-white lysozyme (HEWL) as a function of the laser pulse energy and pulse time width. This is the first recorded study to confirm that the femtosecond laser-induced nucleation of HEWL can be induced at a specific threshold laser energy. The threshold energy is comparable to that of cavitation bubble generation. The results strongly suggest that morphological changes in the solution are key factors for protein nucleation. |
| Keywords: | KeywordHeading" >PACS 81.10.Dn 42.62.Be 87.15.Nt 64.60.Q- 42.65.Re |
| 本文献已被 SpringerLink 等数据库收录！ | |