Study on the Interaction Between Bovine Serum Albumin and Potassium Perfluoro Octane Sulfonate |
| |
Authors: | Ling Li Gong Wu Song Zu Shun Xu |
| |
Affiliation: | 1. Ministry of Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University , Wuhan, Hubei, P. R. China waitingll@yahoo.com;3. Ministry of Education Key Laboratory for the Synthesis and Application of Organic Function Molecules, Hubei University , Wuhan, Hubei, P. R. China |
| |
Abstract: | The interactions between potassium perfluorooctanesulfonate (PFOS) and bovine serum albumin (BSA) were studied by fluorescence spectroscopy. The association constants between PFOS and BSA were obtained by fluorescence enhancing and fluorescence quenching respectively. Furthermore, fluorescence quenching was studied at different temperatures, and the binding constant was also determined by the method of fluorescence quenching. According to the thermodynamic parameters, the main binding force could be judged. The experimental results revealed that BSA and PFOS had strong interactions. The mechanism of quenching belonged to dynamic quenching and the main sort of binding force was hydrophobic force. IR-spectra proved the interaction changed the conformation of BSA. |
| |
Keywords: | BSA energy transfer fluorinated surfactant interaction quenching |
|
|