Study on the Activity and Kinetic Characteristics of Glucoamylase in W/O Microemulsion Systems |
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| Authors: | Xuezhen Ren Ganzuo Li HongLi Wang Yanlian Guan |
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| Affiliation: | 1. School of Chemistry. Shandong University , Jinan, 250100, China;2. lanzou Institute of Chemical Physics, Acdemia Sinica , Lanzou, 730000, China |
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| Abstract: | The activity and kinetic characteristics of glucoamylase has been investigated in W/0 microemulsion systems of AOT/isooctane/buffer, CTAB/isooctane/l-pentanol/buffer and TX-100/ibooctane/I-pentanol/buffer, and compared with that in aquous solution. The effect of various parameters, such as pH optimum, To ptimum and water content, on the activity of the enzyme in microemulsion was determined. The results obtained show that the structure of the microemulsion has strong effect on enzyme activity compared to the kcat, in aquous solution, the apparent turnover number k'cat values were decreased in AOT, TX- 100 based system, and in creased in CTAB based system. |
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| Keywords: | Microemulsions AOT CTAB Triton X-100 Glucoamylase |
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