In Vitro Ultrasound Influence on Cutaneous Permeation of Hyaluronidase |
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Authors: | Vivian Zague Maria Luíza Ozores Polacow Maria Sílvia Mariani Pires-de-Campos Gislaine Ricci Leonardi |
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Affiliation: | 1. University of S?o Paulo, Faculty of Pharmaceutical Sciences , S?o Paulo, Brazil;2. Faculty of Health Sciences, Methodist University of Piracicaba , Piracicaba, Brazil |
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Abstract: | In vitro skin permeation of hyaluronidase enzyme and the ultrasound (US) effects on cutaneous permeation of this enzyme were investigated. Diffusion cell technique, porcine skins, buffer solution, and a hydrophilic gel + hyaluronidase (100 TUR/g), were used for study. The experimental groups were: gel + enzyme and gel + enzyme + US. The activity of permeated hyaluronidase was determined by turbydimetric method in spectrophotometer at 400 nm. Gel + enzyme group permitted a diffusion of 26.89 ± 3.82 TUR of hyaluronidase in the total time of 240 minutes. However, the other group did not produce significant differences (p < 0.05) in the same period (13.68 ± 5.35 TUR). Hyaluronidase permeation through the skin was verified; however US did not cause increase of this enzyme permeation. |
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Keywords: | Hyaluronidase in vitro cutaneous permeation ultrasound |
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