In Vitro Ultrasound Influence on Cutaneous Permeation of Hyaluronidase

In vitro skin permeation of hyaluronidase enzyme and the ultrasound (US) effects on cutaneous permeation of this enzyme were investigated. Diffusion cell technique, porcine skins, buffer solution, and a hydrophilic gel + hyaluronidase (100 TUR/g), were used for study. The experimental groups were: gel + enzyme and gel + enzyme + US. The activity of permeated hyaluronidase was determined by turbydimetric method in spectrophotometer at 400 nm. Gel + enzyme group permitted a diffusion of 26.89 ± 3.82 TUR of hyaluronidase in the total time of 240 minutes. However, the other group did not produce significant differences (p < 0.05) in the same period (13.68 ± 5.35 TUR). Hyaluronidase permeation through the skin was verified; however US did not cause increase of this enzyme permeation.