Simultaneous Determination of Bifonazole and Benzyl Alcohol in Pharmaceutical Formulations by Reverse-Phase HPLC |
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Authors: | O A Čudina M I Čomor I A Janković |
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Institution: | (1) Institute of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmacy, Vojvode Stepe 450, P.O. Box 146, 11000 Belgrade, Serbia and Montenegro;(2) Laboratory for Radiation Chemistry and Physics, Vin a Institute of Nuclear Sciences, P.O. Box 522, 11001 Belgrade, Serbia and Montenegro |
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Abstract: | A simple, precise and sensitive reverse-phase high performance liquid chromatographic (RP-HPLC) method has been developed for the quantitation of bifonazole, an imidazole antifungal, simultaneously with benzyl alcohol, used as preservative, in pharmaceutical formulations. Method employed Zorbax Eclipse XDB-C18 (250×4.6 mm i.d., 5 m) column, methanol - ammonium acetate (pH 2; 65 mM) (65:35, v/v, pH* 3.6) as mobile phase with flow rate of 1 mL min–1 and variable UV detection at 220 and 252 nm. The proposed method was validated by testing its linearity, selectivity, recovery, repeatability, LOD/LOQ values and it was successfully employed for the determination of bifonazole and benzyl alcohol in pharmaceutical cream-based formulations. |
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Keywords: | Column liquid chromatography Pharmaceutical formulations Benzyl alcohol Bifonazole |
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