| Triton X-100敏化鱼精蛋白共振能量转移荧光猝灭法测定尿中8-羟基脱氧鸟苷 |
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| 引用本文： | 米贤文,王永生,薛金花,欧阳运富,杨胜园,王英. Triton X-100敏化鱼精蛋白共振能量转移荧光猝灭法测定尿中8-羟基脱氧鸟苷[J]. 光谱学与光谱分析, 2007, 27(8): 1570-1573 |
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| 作者姓名： | 米贤文 王永生 薛金花 欧阳运富 杨胜园 王英 |
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| 作者单位： | 南华大学公共卫生学院,湖南,衡阳,421001;怀化医学高等专科学校,湖南,怀化,418000;南华大学公共卫生学院,湖南,衡阳,421001;南华大学化学化工学院,湖南,衡阳,421001;南华大学公共卫生学院,湖南,衡阳,421001 |
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| 摘 要： | 研究了8-羟基脱氧鸟苷与鱼精蛋白之间的共振能量转移的最佳条件,建立了鱼精蛋白共振能量转移荧光猝灭法测定8-羟基脱氧鸟苷的新方法.在301 nm,pH 8.2的Tris-HCl缓冲溶液中,鱼精蛋白的荧光猝灭强度与8-羟基脱氧鸟苷的浓度成正比,工作曲线的线性范围为0.50～7.1 μg·mL-1,r=0.996 7,方法的检出限为0.18 μg·mL-1,RSD=6.2%,平均加标回收率为104%(n=6).
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| 关 键 词： | 8-羟基脱氧鸟苷 鱼精蛋白 荧光猝灭 共振能量转移 |
| 文章编号： | 1000-0593（2007）08-1570-04 |
| 修稿时间： | 2006-06-27 |
Determination of 8-Hydroxy-2'-Deoxyguanosine in Urine with Protamine Using Resonance Energy Transfer Fluorescence Quenching by Sensitizing Effect of Triton X-100 |
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| MI Xian-wen,WANG Yong-sheng,XUE Jin-hua,OUYANG Yun-fu,YANG Sheng-yuan,WANG Ying. Determination of 8-Hydroxy-2'-Deoxyguanosine in Urine with Protamine Using Resonance Energy Transfer Fluorescence Quenching by Sensitizing Effect of Triton X-100[J]. Spectroscopy and Spectral Analysis, 2007, 27(8): 1570-1573 |
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| Authors: | MI Xian-wen WANG Yong-sheng XUE Jin-hua OUYANG Yun-fu YANG Sheng-yuan WANG Ying |
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| Affiliation: | 1. College of Public Health, Nanhua University, Hengyang 421001, China 2. College of Chemistry and Chemical Engineering, Nanhua University, Hengyang 421001, China 3. Huaihua Medical College, Huaihua 418000, China |
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| Abstract: | |
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| Keywords: | 8-Hydroxy-2'-deoxyguanosine Protamine Fluorescence quenching Resonance energy transfer |
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