Thermodynamicinvestigation of salting effect of ovalbumin in various salts solutions |
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Authors: | Malgorzata Wszelaka-Rylik W. Zielenkiewicz |
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Affiliation: | (1) Department of Chemistry, Zhejiang University, Hangzhou, 310027, People’s Republic of China;(2) Department of Chemistry and Chemistry Engineering, Zao Zhuang University, Zao Zhuang, 277160, People’s Republic of China; |
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Abstract: | Calorimetric and volumetric investigations of ovalbumin salting by sodium chloride, lithium chloride, potassium chloride, lithium sulfate and ammonium sulfate buffered solutions in the range of concentrations of about 0.2–1.2 M of salts are reported. The evolution of enthalpies and apparent molar volumes vs. salts concentration was investigated using ITC MicroCal titration microcalorimeter and Anton Paar DMA 60/602 digital densimeter. It was found that the changes in enthalpies of salting and apparent molar volumes follow a similar trend. Conclusions about the ability of salts: to precipitate ovalbumin solution are presented. |
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