Rapid method for the determination of amino acids in serum by capillary electrophoresis |
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Authors: | She Zuojun Sun Zimin Wu Lin Wu Kui Sun Siqin Huang Zunbo |
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Affiliation: | Kraft Foods North America, Research and Development, Glenview, IL 60025, USA. lwest@kraft.com |
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Abstract: | Ion-pair and hydrophilic interaction chromatographies are considered to be complementary methods of choice for analyzing intact glucosinolates from broccoli. Ion-pair chromatography resolves non-polar glucosinolates, such as those containing indole moieties, while hydrophilic interaction chromatography is superior for separating polar glucosinolates, such as glucoraphanin and glucoiberin. Reversed-phase separations using hydrophilic endcapped C18-bonded silica and a 50 mM ammonium acetate-methanol gradient mobile phase resolve both polar and non-polar glucosinolates negating the need for switching columns. |
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